Isolation and Identification of Wild Yeasts from Freshwaters and Soils of Nakdong and Yeongsan River,Korea, with Characterization of Two Unrecorded Yeasts

Sang-Min  Han1   Ha-Kun  Kim1   Hyang-Burm  Lee2   Jong-Soo  Lee1,*   

1Department of Biomedicinal Science and Biotechnology, Paichai University
2Division of Food Technology, Biotechnology and Agrochemistry, College of Agriculture and Life Sciences

Abstract

Diverse wild yeast were isolated from freshwaters and soils of Nakdong and Yeongsan rivers in Korea and identified by the comparison of polymerase chain reaction-amplified nucleotide sequences of the internal transcribed spacer region (including the 5.8S rRNA) and D1/D2 regions of 26S rDNA, using BLAST. In total, 15 strains belonging to 9 species were isolated from 25 samples, out of which Aureobasidium pullulans and Cryptococcus bestiolae were dominant. Candida ghanaensis JSF0127 and Meira geulakonigii JSF0130 were identified as unrecorded yeasts, for which their mycological characteristics were investigated. These unrecorded yeasts formed ascospores and grew in yeast extract peptone dextrose medium containing 5% NaCl.

Figures & Tables

Sampling sites in Yeongsan river (A) and Nakdong river (B), Korea.