Notes on Five Unrecorded Endophytic Fungi Isolated from Coniferous Leaves and Orchid Roots in Korea

Hyeok  Park1   Bong-Hyung  Lee1   Yu-Ra  Bae1   Dong-Yeo Kim1   Ahn-Heum  Eom1,*   

1Department of Biology Education, Korea National University of Education

Abstract

We collected leaves of Pinus koraiensis and Thuja koraiensis and roots of Bletilla striata from various sites in Korea. The leaf and root samples were surface-sterilized and endophytic fungi were isolated. Fungal isolates were identified based on their morphological characteristics and a phylogenetic analysis of internal transcribed spacer regions, large subunit regions, and the β-tubulin gene. Consequently, we identified five species of endophytic fungi, namely Colletotrichum simmondsii, Fusarium sterilihyphosum, Diatrypella pulvinata, Ochroconis globalis, and Sphaeria chrysosperma. These species have not been previously reported in Korea and we report them here with descriptions and illustrations.

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