Development of Cleaved Amplified Polymorphic Sequence Markers for the Identification of Lentinula edodes Cultivars Sanmaru 1ho and Chunjang 3ho

Suyun  Moon1   Hwa-Yong  Lee12   Myungkil  Kim3   Kang-Hyeon  Ka3   Han Kyu  Ko4   Jong-Wook  Chung5   Chang-Duck  Koo2,*   Hojin  Ryu1,*   

1Department of Biology, Chungbuk National University
2Department of Forest Science, Chungbuk National University
3Wood Chemistry and Microbiology Division, National Institute of Forest Science
4Forest Mushroom Research Center, National Forestry Cooperative Federation
5Department of Industrial Plant Science and Technology, Chungbuk National University

Abstract

Lentinula edodes is an edible mushroom that is mainly cultivated in Asian countries. Recently, new cultivars of this mushroom have been developed in Korea; variety protection is very important, so the development of efficient molecular markers that can distinguish each variety is required. In this study, we developed cleaved amplified polymorphic sequence (CAPS) markers for the identification of L. edodes cultivars (Sanmaru 1ho and Chunjang 3ho). These markers were developed from whole genomic sequencing data from L. edodes monokaryon strain B17 and resequencing data from 10 dikaryon strains. A single nucleotide polymorphism changed in scaffold 9 POS 1630048 in Sanmaru 1ho(G→T), and in scaffold 13 POS 920681 in Chunjang 3ho (G→A). The restriction enzymes TspR I and Xho I distinguished Sanmaru 1ho and Chunjang 3ho, respectively, from other strains. Thus, we developed 2 CAPS markers for the identification of the L. edodes cultivars Sanmaru 1ho and Chunjang 3ho

Acknowledgements

This study was supported by a grant Golden Seed Project (Center for Horticultural Seed Development, No. 213007-05-1-SBH20).

Figures & Tables