Confirmation of Chestnut Powdery Mildew, Erysiphe castaneigena, in Korea with Morphological Characteristics and Molecular Analyses

Sung-Eun  Cho1   Sang-Hyun  Lee2    Sun Keun  Lee3   Hyeon-Dong  Shin1,*   

1Division of Environmental Science and Ecological Engineering, Korea University
2Warm-Temperate and Subtropical Forest Research Center, National Institute of Forest Science
3Division of Forest Diseases and Insect Pests, National Institute of Forest Science

Abstract

The powdery mildew on Castanea crenata in South Korea was first recorded as Microsphaera alni in 1958. On the basis of its morphological characteristics and host range, the mildew was determined as Microsphaera sinensis in 1988. According to the rules of The International Code of Botanical Nomenclature, M. sinensis was renamed Erysiphe castaneigena in 2006. Nevertheless, taxonomic re-consideration of the morphological and molecular characteristics for the Castanea powdery mildew in Korea has not been performed. In the present study, we studied 34 powdery mildew samples collected from C. crenata in the Korea University Herbarium. On the basis of microscopic examinations of the holomorph and phylogenetic analysis of the internal transcribed spacer regions, we confirmed the powdery mildew fungus as E. castaneigena. In addition, sequence comparison between E. castaneigena and E. alphitoides ex Castanea sp. suggested a close phylogenetic affinity.

Acknowledgements

This work was financially supported by a grant from the National Institute of Forest Science. Additional funding was received by a grant from Korea University to HDS.

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