Development of a Segregating Population with Biological Efficiency in Agaricus bisporus

Youn-Lee  Oh1,*   Anton S. M.  Sonnenberg2   Johan J. P.  Baars2   Kab-Yeul  Jang2   Min ji  Oh1   Ji-Hoon  Im1   Won-Sik  Kong1   

1Mushroom Science Division, National Institute of Horticultural and Herbal Science
2Plant Breeding, Wageningen University and Research

Abstract

In this study, we made a population with high biological efficiency (BE) to investigate the complex genetic architecture of yield-related traits in Agaricus bisporus. MB-013 crossed between bisp 015-p2 and bisp 034-p2, had high BE. Additionally MB-013 was an intervarietal hybrid that intercrosses with A. bisporus var. burnettii, bisp 015, and A. bisporus var. bisporus, bisp 034. One hundred and seventy homokaryons were selected using the cleaved amplified polymorphic sequence (CAPS) markers (PIN primer/HaeIII) from 300 single spore isolates (SSIs). One hundred BC1F1 hybrids were obtained by crossing the homokaryons of MB-013 with bisp15-p1. The population of 100 BC1F1 hybrids is suitable for analyses of BE.

Acknowledgements

This study was supported by the National Institute of Horticultural & Herbal Science, Republic of Korea and Plant Research International (PPO/PRI) established in Wageningen, Netherlands through the international cooperative research project ‘Development of the breeding technology and molecular markers based on mushroom genome’ funded by the Rural Development Administration (PJ012056).

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