Anti-inflammatory Activity of Wild Yeasts, Meyerozyma guilliermondii YJ34-2 and Rhodotorula graminis YJ36-1, on Lipopolysaccharide-induced Nitric Oxide in RAW 264.7 Cells Through the Inhibition of Nitric Oxide and Cytotoxic Effects

Sang-Min  Bae1   Sang-Min  Han1   Jong-Soo  Lee1,*   

1Department of Biomedicinal Science and Biotechnology, Paichai University

Abstract

The anti-inflammatory effects of cell-free extracts from wild yeasts, Meyerozyma guilliermondii YJ34-2 and Rhodotorula graminis YJ36-1, caused by the inhibition of nitric oxide (NO) activity and cytotoxic effects were determined. Cell-free extracts from these two yeast strains had dose-dependent inhibitory effects on the production of lipopolysaccharide-induced NO and there were no cytotoxic effects on the treated cells or negative effects on their proliferation. Their cell-free extracts were also shown to have inhibitory effects on pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α and prostaglandin-E2, in a dose-dependent manner. Maximal inhibitory activity on NO production occurred in cell-free extracts of Meyerozyma guilliermondii YJ34-2 cultivated at 30°C for 24 hr and Rhodotorula graminis YJ36-1 cultivated at 25°C for 24 hr in the yeast extract-peptone-dextrose (YPD) media.

Acknowledgements

This work was supported by the research grant of Pai Chai University in 2017.

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