Phylogenetic Analysis of Downy Mildew Caused by Peronospora destructor and a Method of Detection by PCR

Chang-Gi  Back1   Sun-Kyung  Hwang2   Mi jeong  Park1   Young-Seok  Kwon3   Hee-Young Jung4,*   Jong-Han  Park1   

1Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science
2Technology Services Division, National Institute of Horticultural and Herbal Science
3Vegetable Research Division, National Institute of Horticultural and Herbal Science
4School of Applied Biosciences, Kyungpook National University

Abstract

Onion downy mildew, caused by Peronospora destructor, is a major disease in onion cultivation areas in Korea. The causal fungi were collected and analyzed based on sequence similarity and molecular phylogenetic relationships of multi-gene sequences, including the internal transcribed spacer (ITS) region. All isolates from Changnyeong-gun, Hamyang-gun, and Hapcheon-gun in Gyeongnam province, and Muan-gun, Haenam-gun, and Sinan-gun in Jeonnam province were identical in the four types of gene sequences, indicating they were genetically the same strains. In this study, a PCR method was developed based on the ITS gene sequences to amplify the specific DNA fragment for P. destructor only. The detection limit of was total genomic DNA of the P. destructor and the plant 0.7 ng/μL. Therefore, the developed PCR method could be used to detect P. destructor effectively from symptomless onion leaves.

Acknowledgements

This work was carried out with the support of “Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ011988012017)” Rural Development Administration, Republic of Korea.

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