Abstract
Three fungal isolates, NNIBRFG108, 1139, and 1480, were isolated from freshwater environments; NNIBRFG108 from plant litter in Samcheok, Gangwon and NNIBRFG1139 and 1480 from the soil in Jeju & Gimcheon, Gyeongbuk, Korea. Based on the morphological characteristics and phylogenetic analysis of internal tanscribed spacer (ITS), 28S rDNA region, and β-tubulin gene, NNIBRFG108, NNIBRFG1139, and NNIBRFG1480 isolates were confirmed as Didymella segeticola, D. ellipsoidea, and D. aeria, respectively. Neither species has previously been described in Korea.
Acknowledgements
This results was supported by a grant from the Nakdonggang National Institute of Biological Resources (NNIBR) of the Republic of Korea for projects on survey and discovery of freshwater bioresources (NNIBR, 2017).
Figures & Tables
Fig. 1. Neighbor-joining tree of a concatenated alignment of internal transcribed spacer (ITS), 18S, 28S rDNA region and β-tubulin of three Didymella species including D. segeticola, D. ellipsoidea and D. aeria from deposited plant litter and soil. Neoascochyta europaea and N. paspali were used as an outgroup. Bootstrap values higher than 50% from 1,000 replications were shown above/below branches.