First Report of Three Didymella Species Isolated from Freshwater Ecosystem in Korea

Hye Yeon Mun1,*   Jaeduk Goh1   Yoosun Oh1   Ae-Ran Jeong1   Namil Chung1   

1Fungal Resources Research Division, Freshwater Bioresources Research Bureau, Nakdonggang National Institute of Biological Resources

Abstract

Three fungal isolates, NNIBRFG108, 1139, and 1480, were isolated from freshwater environments; NNIBRFG108 from plant litter in Samcheok, Gangwon and NNIBRFG1139 and 1480 from the soil in Jeju & Gimcheon, Gyeongbuk, Korea. Based on the morphological characteristics and phylogenetic analysis of internal tanscribed spacer (ITS), 28S rDNA region, and β-tubulin gene, NNIBRFG108, NNIBRFG1139, and NNIBRFG1480 isolates were confirmed as Didymella segeticola, D. ellipsoidea, and D. aeria, respectively. Neither species has previously been described in Korea.

Acknowledgements

This results was supported by a grant from the Nakdonggang National Institute of Biological Resources (NNIBR) of the Republic of Korea for projects on survey and discovery of freshwater bioresources (NNIBR, 2017).

Figures & Tables

Fig. 1. Neighbor-joining tree of a concatenated alignment of internal transcribed spacer (ITS), 18S, 28S rDNA region and β-tubulin of three Didymella species including D. segeticola, D. ellipsoidea and D. aeria from deposited plant litter and soil. Neoascochyta europaea and N. paspali were used as an outgroup. Bootstrap values higher than 50% from 1,000 replications were shown above/below branches.