Isolation and Characterization of Wild Yeasts from Water and Riverside Soils of Geumgang Midstream in Gongju City, Korea

Sang-Min Han1   Ji-Yoon Kim1   Hyang Burm Lee2   Ha-Kun Kim1   Jong-Soo Lee1,*   

1Department of Biomedicinal Science and Biotechnology, Paichai University
2Division of Food Technology, Biotechnology and Agrochemistry, College of Agriculture and Life Science, Chonnam National University

Abstract

The goal of this study was to elucidate wild yeast diversity of the midstream region of the Geumgang river, Gongju, Chungnam province, Korea. Fifty strains of 24 species of wild yeasts were isolated from 45 water and soil samples among which Cryptococcus spp. (11 strains), Cryptococcus magnus (7 strains), Rhodotorula spp. (9 strains), and Hanseniaspora spp. (6 strains) were dominant. Four species, Candida chauliodes WJSF 0201, Candida oleophila WJSF 0202, Candida catenulata WJSF 0203, and Candida jaroonii WJSF 0204, represented newly recorded yeasts in Korea. All of these unrecorded yeasts were oval in shape, formed ascospores and pseudomycelium, and grew in vitamin-free medium. Candida oleophila WJSF 0202 was thermophilic which can grow at 37°C.

Acknowledgements

This work was supported by a grant from the Nakdonggang National Institute of Biological Resources (NNIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea.

Figures & Tables

Fig. 2. Phylogenetic tree of the unrecorded yeasts isolated from water and riverside soils of Geumgang midstream in Gongju city, Korea, based on the nucleotide sequences of large subunit 26S ribosomal DNA D1/D2 region. The tree was generated by the neighbor-joining method, using MEGA7.