Aspergillus caninus (Syn: Phialosimplex caninus): A New Record from Field Soils in Korea

Mahesh Adhikari1   Sun Kumar Gurung1   Sang Woo Kim1   Hyun Goo Lee1   Han Jun Ju1   Byeong Heon Gwon1   San Kosol1   Setu Bazie1   Hyang Burm Lee2   Youn Su Lee1   

1Division of Biological Resources Sciences, Kangwon National University, Chuncheon 24341, Korea
2Division of Food Technology, Biotechnology and Agrochemistry, Chonnam National University, Gwangju 61186, Korea

Abstract

During the study of indigenous fungal communities in soil samples collected from various field soils in Sancheong, Gyeongsangnam-do, Korea in 2017, several species of Aspergillus caninus were discovered. Aspergillus caninus (KNU17-7) was isolated, identified, and described based on the results from macro and micro morphological characteristics and molecular characterization. Morphologically, it was identified using five different growth media: potato dextrose agar, oatmeal agar, yeast extract sucrose agar, czapek yeast extract agar, and malt extract agar. For the molecular identification, sequencing of internal transcribed spacer, β-tubulin, and calmodulin genes was performed. Based on this characterization, our study isolate was identified as Aspergillus caninus. This fungal species has not been officially reported in Korea before, and we report here with its morphological and molecular phylogenetic characterization.

Acknowledgements

This work was carried out with the support of National Institute of Biological Resource (NIBR), Golden Seed Project (Center for Horticultural Seed Development, No.213007-05-2-WTH32), Ministry of Agriculture, Food and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOF), Rural Development Administration (RDA) and National Institute of Horticultural and Herbal Science (PJ01328102), Rural Development Administration, Republic of Korea.

Figures & Tables

Fig. 1. Morphological features of the KNU17-7 colony grown for 7 days on potato dextrose agar (PDA), oatmeal agar (OMA), yeast extract sucrose agar (YESA), malt extract agar (MEA), and Czapek yeast extract agar (CYEA) at 25°C. A, F, front and back side of the colony on PDA; B, G, front and back side on OMA; C, H, front and back side on YESA; D, I, front and back side on MEA; E, J, front and back side on CYEA.