Production of Anti-dementia Acetylcholinesterase Inhibitors from the Wild Yeasts Saccharomyces cerevisiae WJSL0113 and Wickerhamomyces anomalus JSF0128

 Ji-Yoon Kim, Sang-Yeop Lee, Sang-Min Han and Jong-Soo Lee*. *   

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Abstract

In this paper, the screening of potent acetylcholinesterase (AChE) inhibitor - producing yeasts from wild yeasts and the condition for the production of anti-dementia AChE inhibitors are described. Among one hundred and seven non-pathogenic wild yeast strains from the waters and soils of three main rivers in Daejeon metropolitan city and midstream of Yeongsangang river in Sangju, sporogenous Saccharomyces cerevisiae WJSL0113 and asporogenous Wickerhamomyces anomalus JSF0128 were selected as useful strains for the production of potent AChE inhibitors. The AChE inhibitors of Saccharomyces cerevisiae WJSL0113 and Wickerhamomyces anomalus JSF0128 had a maximum yield when they were incubated in yeast extract-peptone-dextrose media (pH 6.0 in Saccharomyces cerevisiae WJSL0113 and pH 5.0 in Wickerhamomyces anomalus JSF0128) for 18 hr at 30℃, respectively.

Acknowledgements

This work was supported by the research grant of PaiChai University in 2018.

Figures & Tables

Fig. 1. Phylogenetic tree of anti-dementia acetylcholinesterase inhibitor-producing yeasts, WJSL0113 and Wickerhamomyces JSF0128, isolated from freshwater based on the nucleotide sequences of large subunit 28S ribosomal DNA. The tree was generated by the neighbor-joining method, using MEGA7