Microbiological Characteristics and Physiological Functionalities of Unrecorded Wild Yeast Strains in the Soils of Hajodae and Gyungpodae Beaches in Korea

Ha-Kun Kim1   Ji-Yoon Kim1   Sang-Min Han1   Changmu Kim2   Jong-Soo Lee1,*   

1Department of Biomedicinal Science and Biotechnology, Paichai University, Daejeon 35345, Korea
2Microorganism Resources Division, National Institute of Biological Resources, Incheon 22689, Korea

Abstract

The goal of this study was to isolate and characterize the wild yeast strains in the soils of Hajodae and Gyungpodae Beaches, Gyungpo Lake in Kangwon-do, Korea and evaluate their anti-demential and tyrosinase inhibitory activities. Among the 27 yeast strains isolated from 30 soil samples from Hajodae Beach, Cyberlindnera culbertsonii HJ31-1, Dothichiza pithyophila HJ22-4, Metschnikowia bicuspidata HJ16-1, and Sakaguchia cladiensis HJ14-1 were recorded for the first time in Korea. Additionally, six of the 55 wild yeast strains isolated from 45 soil samples from Gyungpodae Beach and Gyungpo Lake had not previously been detected in Korea, such as Aureobasidium melanogenum JR3-1. The microbiological characteristics and anti-demential and tyrosinase inhibitory activities of these previously unrecorded yeasts were investigated. Almost all of them were oval-shaped and had ascospores except for Dothichiza pithyophila HJ22-4, Diutina siamensis JR37-5, and Canadida gelsemii DC 35-1. Furthermore, they were all sugar-tolerant and able to grow in 20% glucose-containing YPD broth, except for Cyberlindnera culbertsonii HJ31-1. Diutina siamensis JR37-5 had the highest tyrosinase inhibitory activity (30.9%).

Figures & Tables

Phylogenetic tree of the unrecorded yeasts isolated from Hajodae and Gyungpodae Beaches in Korea, based on the nucleotide sequences of D1/D2 region of 26S ribosomal. The tree was generated by the neighbor-joining method, using MEGA7.