Morphological and Phylogenetic Characteristics of Tuber himalayense Collected from Rhizosphere of Quercus dentata in Korea

Hyeok Park1   Ju-Hui Gwon1   Jong-Chul Lee1   Hyun Suk Kim2   Geon-Sik Seo3   Ahn-Heum Eom1,*   

1Department of Biology Education, Korea National University of Education, Cheonju 28173, Korea
2Jellanamdo Forest Resources Research Instutute, Naju 58213, Korea
3Department of Mushroom Science, Korea National College of Agriculture and Fisheries, Jeonju 54874, Korea

Abstract

We collected the ascomata of Tuber species from the rhizosphere of Quercus dentata in Danyang, Korea. We observed the morphological characteristics of ectomycorrhizal roots and ascomata, and identified the species based on the results of the phylogenetic analysis conducted using the DNA sequences of an internal transcribed spacer, a large-subunit rDNA, translation elongation factor 1-α DNA (TEF1), and MAT. Finally, we identified the fungal species as Tuber himalayense B.C. Zhang & Minter, which has not been recorded previously in Korea. We evaluated the morphological characteristics and conducted phylogenetic analysis of the ascoma and mycorrhiza (associated with Q. dentata) of T. himalayense.

Figures & Tables

Fig. 1. Morphological characteristics of . Ascoma (A, B), surface ornament (C), peridium and gleba (D) asci (E, F), and ascospores (G, H) Scale bars: B=5,000 μm, C, D=2,000 μm, E=50 μm, F=20 μm, G, H=10 μm.