Screening of Antagonistic Bacteria having Antifungal Activity against Various Phytopathogens

Hee-Jong Yang1   Su-Ji Jeong1   Seong-Yeop Jeong1   Do-Youn Jeong1   

Abstract

The aim of this study was to isolate a potential multifunctional biocontrol agent from bacteria for control of multiple plant diseases as an alternative to fungicides. A total of 201 strains were isolated from soil undamaged by repeated cultivation in Sunchang and their ability to produce antibiotics, siderophores and extracellular enzymes such as protease, cellulase and amylase was investigated. Selected strain SCS3 produced cellulose, protease and amylase. This strain also produced siderophores and showed excellent antifungal activity against various phytopathogens. SCS3 was identified as Bacillus subtilis using 16S rRNA sequencing, and named Bacillus subtilis SCS3. Finally, physiological and biochemical characteristics of B. subtilis SCS3 were examined. From the results, B. subtilis SCS3 was found to be a useful multifunctional biocontrol agent against various phyto- pathogens.

Acknowledgements

본 연구는 농촌진흥청 공동연구사업(과제번호: PJ009990)의 지원에 의해 이루어진 것입니다.

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