Plant Growth Promotion and Gibberellin A3 Production by Aspergillus flavus Y2H001

Young-Hyun You1,*   Jong Myong Park2   Sang-Mo Kang3   Jong-Han Park1   In-Jung Lee3   Jong-Guk Kim4,*   

Abstract

Perilla frutescens var. japonica Hara was collected from farmland in Seongju-gun. Fifteen endophytic fungal strains with different colony morphologies were isolated from roots of P. frutescens. Waito-c rice seedlings were treated with the concentrated culture filtrates (CF) of endophytic fungi for observation of their plant growth-promoting activities. In the results, the CF of Y2H001 fungal strain promoted the growth of the waito-c rice seedlings. The phylogenetic tree of Y2H001 strain was analyzed by the combined sequences of the partial internal transcribed spacer region (ITS) and partial betatubulin gene. Molecular and morphological studies identified the Y2H001 strain as belonging to Aspergillus flavus. In gas chromatography mass spectrometry (GC/MS) analysis of the CF of Y2H001 strain, gibberellic acid (GA) was detected and quantified. Therefore, we describe Y2H001 strain as a new GA3-producing A. flavus based on morphological, molecular characteristics and analysis of secondary metabolite.

Acknowledgements

This work was carried out with the support of “Research Program for Agriculture Science & Technology De- velopment (Project title: Diagnosis of horticultural and herbal crops diseases and insect pests, Project No. PJ011 36802)” Rural Development Administration, Republic of Korea.

Figures & Tables