Isolation and Identification of Yeasts from Jeju Island Soils

Sang-Min Han1   Sang-Min Bae1   Jae-Won Han1   Ji-Yoon Kim1   Jong-Soo Lee1   Ha-Kun Kim1,*   

Abstract

Significant differences in annual precipitates on Jeju island have been reported depending on the location. We collected soil samples from east and west areas of Jeju Island to identify yeasts by plating on yeast peptone dextrose plates and subsequent analysis for the polymerase chain reaction amplified D1/D2 region of 26S rDNA of colonies. As a result, 20 yeast strains of 12 species were isolated from 7 different sampling sites in east area and 13 yeast strains of 6 species from 5 different sampling sites in west area. Some differences in yeast flora were observed depending on the sample collection sites having different annual precipitates.

Acknowledgements

This work was funded by the project on survey and excavation of Korean indigenous species of National Institute of Biological Resources (NIBR) under the Ministry of Environment.

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