Mycelial Growth Properties of Domestically Collected Ectomycorrhizal Tricholoma Mushrooms in Various Culture Conditions

 강정아1,3    가강현2    김준영1,3    김성환*1,3   

1Department of Microbiology, College of Natural Science, Dankook University, Cheonan 31116, Korea
2Special-purpose Trees Division, National Institute of Forest Science, Suwon 16631, Korea
3Institute of Biodiversity, Dankook University, Cheonan 31116, Korea

Abstract

The ectomycorrhizal basidiomycete Tricholoma is one of mushroom groups that cannot be cultivated artificially. To use this mushroom as applicable resource for food production, it is necessary to obtain information about their mycelial growth properties in various environmental conditions. This study investigated the mycelial growth of four domestic isolates of Tricholoma species (T. bakamatsutake, T. fulvocastaneum, T. matsutake, T. terreum) at different physical and chemical conditions. The optimal physical conditions for their mycelia growth were found to be a temperature range of 20~25℃ and a pH range of 4.0~7.0 in dark condition. The growth of T. matsutake was retarded at high temperature (30℃). Tests to determine the chemical factors that affected mycelial growth showed that the four Tricholoma spp. grew 1% saline. T. matsutake grew in up to 2% saline. In the presence of various heavy metals (50 ppm) and pesticides (suppliers’ recommended concentration), mycelial growth was inhibited the most by cadmium and emamectin benzoate, respectively. However, all the four Tricholoma spp. grew with Cu+. The growth of T. matsutake was not inhibited by abamectin, acetamiprid, and thiacloprid. Extracellular enzyme activities of amylase and β-glucosidase were detected only in T. bakamatsutake and T. fulvocastaneum. The results of the present study allowed us to determine suitable or harmful environmental conditions for the mycelial cultivation of the Tricholoma spp.

Figures & Tables

Fig. 1. Photos of the fruiting bodies of the four species collected in Korea and employed for the isolation of the cultures used for this study. A,; B,; C,; D,