Suitable Conditions for Mycelial Culture of Tremella fuciformis

Eun-ji Lee1   Hye-Sung Park1   Chan-Jung Lee1   Won-Sik Kong1   Chang-Duck Koo2,*   

1Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA
2Department of Forestry Science, Chungbuk National University

Abstract

This study was conducted to investigate the optimum culture conditions, for mass production of Tremella fuciformis in M9 basic medium. The strain KMCC04674, used in this study was identified T. fuciformis by internal transcribed spacer (ITS). To define the optimum conditions for the mass production of T. fuciformis, we investigated the effects of different culture conditions and various nutrient sources on the fungal growth. The optimum initial pH and temperature for the fungal growth were 5.0 and 25℃, respectively. The optimal composition of the growth medium was 4.0% mannitol, 3.0% NH4H2PO4, 1.0% malt extract, 1.0% glutamic acid, 5mM CaCl2, and 0.5% glucanic acid.

Figures & Tables

Fig. 1. Phylogenetic relationships of based on internal transcribed spacer(ITS) sequences. Numerical values on branches are the bootstrap values as percentage of bootstrap replication from 1000 replicate analysis. Bar = 0.02 genetic distance between samples.