Three Unreported Endophytic Fungi Isolated from Conifer Leaves of Pinus densiflora in Korea

Hyeok Park¹ Ahn-Heum Eom^1,*

¹Department of Biology Education, Korea National University of Education

Abstract

We isolated endophytic fungi from the conifer leaves of Pinus densiflora inhabiting Buan-gun, Jeollabuk-do, Korea. We identified the isolated fungal strains based on phylogenetic analysis performed using the nucleotide sequences of the internal transcribed spacer, large subunit, and beta-tubulin. We confirmed the presence of three novel endophytic fungi in Korea, namely Paracamarosporium hawaiiense, Tubakia dryina, and Zasmidium fructigenum. In this report, we described the morphological characteristics of these fungal strains and the results of their phylogenetic analysis.

Figures & Tables

Fig. 1. Colonies of 16B647 grown for 7 days on potato dextrose agar (A) and malt extract agar (E), conidia (D, H). Colonies of (16B554) grown for 7 days on PDA (B) and MEA (F), conidiophore and conidia (I, J). Colonies of (16B210) grown for 7 days on PDA (C) and MEA (G), conidiophore and conidia (K, L). (scale bars =10 μm).