Gibberellins Production and Identification of Endophytic Fungi Isolated from Aquatic Plant in Fresh Water

Young-Hyun You1,3   Sang-Mo Kang2   Yu-Mi Choi3   Myung-Chul* Lee3   Jong-Guk* Kim1   

Abstract

ABSTRACT : Aquatic plant Hydrocharis dubia (Blume) Backer was collected from the Dalsung wetland in Daegu. Sixteen endophytic fungi with different colony morphologies were isolated from the roots of aquatic plants. Waito-c rice (WR) seedlings were treated with fungal culture filtrates (FCF) for screening plant growth-promoting activity. In the results, HD1008 strain isolated from aquatic plant showed highest plant growth-promoting activity. The FCF of HD1008 strain was analyzed using gas chromatography mass spectrometry (GC/MS) with selected ion monitoring (SIM). Analysis of the FCF of HD1008 strain found that it contained gibberellins (GA) (GA1, 1.2 ng/100 mL; GA4, 5 ng/100 mL). Phylogenetic tree of HD1008 strain was constructed by partial internal transcribed spacer (ITS) region and partial beta-tubulin gene sequences. Therefore, we describe HD1008 strain as a new gibberellin-producing Penicillium trzebinskii based on morphological and molecular characteristics.

Acknowledgements

This study was supported by a grant (PJ01010602) from National Academy of Agricultural Science, RDA, Republic of Korea.

Figures & Tables