A fungal strain designated KNU-WDM2A2 was isolated from mosquitoes in Gimcheon, Korea. The pure culture was transferred to potato dextrose agar (PDA) and synthetic nutrient agar (SNA) media and attained a diameter of 90 mm after 10 days of incubation at 25℃. The colonies were whitish to light pink and cottony to wooly, with an abundant production of aerial mycelia. The strain produced hyaline to slightly yellowish conidiophores that were rough-walled and branched, with conidiogenous cells arising terminally or laterally. Conidia were unicellular, hyaline to light brown, smooth, and oval or ovoid to clavate, with a size of 4.1-6.9×2.5-3.3 μm (n=65). A phylogenetic analysis was conducted using the internal transcribed spacer (ITS) regions and 28S rDNA of large subunit (LSU) sequences, to support the cultural and morphological characteristics. The KNU-WDM2A2 strain was identified here as
Figures & Tables
Cultural and morphological characteristics of KNU-WDM2A2. Colonies on potato dextrose agar (A) and synthetic nutrient agar (B) after 14 days of inoculation at 25℃; Conidiomata observed under a stereomicroscope (C); Conidiophores (D-E); Conidiogenous cells (F); Conidia (G). The arrows indicate conidiogenous cells. Scale bars: C=50 μm; D-G=10 μm.