A New Report of Biscogniauxia petrensis Isolated from Mosquitoes in Korea

Kallol Das1   Joung-Ho  Kim1   Kwang-Shik  Choi2   Seung-Yeol  Lee1,3   Hee-Young  Jung1,3,*   

1School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea
2School of Life Sciences, Kyungpook National University, Daegu 41566, Korea
3Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Korea

Abstract

A fungal strain designated KNU-WDM2A2 was isolated from mosquitoes in Gimcheon, Korea. The pure culture was transferred to potato dextrose agar (PDA) and synthetic nutrient agar (SNA) media and attained a diameter of 90 mm after 10 days of incubation at 25℃. The colonies were whitish to light pink and cottony to wooly, with an abundant production of aerial mycelia. The strain produced hyaline to slightly yellowish conidiophores that were rough-walled and branched, with conidiogenous cells arising terminally or laterally. Conidia were unicellular, hyaline to light brown, smooth, and oval or ovoid to clavate, with a size of 4.1-6.9×2.5-3.3 μm (n=65). A phylogenetic analysis was conducted using the internal transcribed spacer (ITS) regions and 28S rDNA of large subunit (LSU) sequences, to support the cultural and morphological characteristics. The KNU-WDM2A2 strain was identified here as Biscogniauxia petrensis, new to Korea.

Figures & Tables

Cultural and morphological characteristics of KNU-WDM2A2. Colonies on potato dextrose agar (A) and synthetic nutrient agar (B) after 14 days of inoculation at 25℃; Conidiomata observed under a stereomicroscope (C); Conidiophores (D-E); Conidiogenous cells (F); Conidia (G). The arrows indicate conidiogenous cells. Scale bars: C=50 μm; D-G=10 μm.