First Report on Lophiostoma macrostomum and Pestalotiopsis lespedezae Isolated from Conifer Leaves of Pinus densiflora in Korea

Hyeok Park1   Ahn-Heum Eom1,*   

1Department of Biology Education, Korea National University of Education, Cheonju 28173, Korea


In this study, we isolated endophytic fungi from the conifer leaves of Pinus densiflora inhabiting Mt. Gyehwasan in Korea. We identified the isolated fungal strains based on their morphological characteristics and phylogenetic analysis using DNA sequences of the internal transcribed spacer, small subunit rDNA, large subunit rDNA, and β-tubulin region. In the process of this study, we confirmed two endophytic fungi that have not been recorded previously in Korea, Lophiostoma macrostomum and Pestalotiopsis lespedezae. In this report, we described the morphological characteristics of these fungal strains and the results of their molecular analysis.

Figures & Tables

Fig. 1. Colonies of strain 16B060 (Lophiostoma macrostomum) grown for 7 days on potato dextrose agar (PDA) (A, E) and malt extract agar (MEA) (B, F); Conidiophore and chlamydospore (I, J). Colonies of strain 16B115 (Pestalotiopsis lespedezae) grown for 7 days on PDA (C, G) and MEA (D, H); conidiophore and conidia (K, L). (scale bars: I, J=20 μm; K, L=10 μm)