Microbiological Characteristics and Carbon source activity of Unrecorded Wild Yeast Strains from gut of the earthworm(

Joo Hyun  Han1   Sang Eun  Lee1   Myung Kyum  Kim1,*   

1Department of Bio & Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul 01767, Republic of Korea

Abstract

The goal of this study was to isolate and characterize the wild yeast strains from the gut of earthworm(Eisenia andrei ). The 19 yeast strains isolated from 5 gut of earthworm samples from Nanji water regeneration center in Goyang-si, Gyeonggi-do, Korea. Among them, 16 strains were recorded and 3 strains, Yarrowia deformans YP242 (=KACC48778), Sporidiobolus pararoseus YP66 (=KCTC27963) and Sporidiobolus pararoseus YI9 (=KACC48948) were recorded for the first time in Korea. The microbiological characteristics of these previously unrecorded yeasts were investigated. All three strains were oval-shaped, convex and smooth. However, they showed some differences in colony color and result of carbon assimilation assays. YP242 was white-colored and assimilated glycerol, L-arabinose and N-acetyl-D-glucosamine as carbon sources. YP66 was red-colored and assimilated D-Saccharose. YI9 was white-colored and positive for 2-keto-D-gluconate assimilation.

Figures & Tables

Fig. 1. Phylogenetic tree of the unrecorded yeasts isolated from gut of the earthworm in Korea, based on the nucleotide sequences of D1/D2 sequence. The tree was generated by the neighborjoining method, using MEGA7. Bar: 0.03