Isolation of Wild Yeasts Obtained from Waters and Soils of Riversides in Sangjubo of Nakdong River and Daechung Dam of Geumgang Midstream, Korea and Characterization of Unrecorded Wild Yeasts

Sang-Min Han1   Dong-Jae Park1   Ji-Yoon Kim1   Hyo Jin Lim2   Hyang Burm Lee2   Jong-Soo  Lee1,*   

1Faculty of Biomedicine and Biotechnology, Paichai University, Daejeon 35345, Korea
2Division of Food Technology, Biotechnology and Agrochemistry, Chonnam National University, Gwangju 61186, Korea


The goal of this study was to isolate wild yeasts from waters and soils in Sangjubo of Nakdong river and Daechung dam in Daejeon city, Korea and investigate characteristics of previously unrecorded novel wild yeasts. In total, 94 strains from 39 different species of wild yeasts were isolated from 47 soils and waters samples obtained from Sangjubo in Nakdong river. Among these, 3 strains, including Rhodosporidium azoricum JSL-GRU-009, represented novel yeast strains which were previous recorded. Two hundred nine strains from 105 different species of wild yeasts were isolated from 130 soils and water samples obtained from Daechungdaegyo and Daechung dam of Shintanjin in Daejeon city, Korea. Ten strains including Bullera pseudoalba JSL-GRU-005 also represented newly recorded strains in Korea. All the 13 previously unrecorded yeasts were oval or global in shape, and nine strains, including R. azoricum JSL-GRU-009, formed ascospores. Ten strains grew well in vitamin-free medium. R. azoricum JSL-GRU-009 grew well in 20% glucose-containing yeast extract-peptone-dextrose (YPD) medium and 3 strains, including Rhodotorula oryzicola JSL-GRU-003, grew well in 5% NaCl-containing YPD medium.

Figures & Tables

Fig. 1. Phylogenetic tree of the unrecorded yeasts isolated from soils of spices plants fields and Mt. Daedun of Chungcheongnam-do, Korea, based on the nucleotide sequences of large subunit 26S ribosomal DNA D1/D2 region. The tree was generated by the neighborjoining method, using MEGA7.