Unrecorded Endophytic Fungi Belonging to Genus Phyllosticta Isolated from Leaves of Woody Plants

Hyeok Park1   Jong-Chul Lee1   Ju-Hui Gwon1   Hyang Burm Lee2   Ahn-Heum Eom1,*   

1Department of Biology Education, Korea National University of Education, Cheongju 28173, Korea
2Department of Agricultural Biological Chemistry, College of Agriculture & Life Sciences, Chonnam National University, Gwangju 61186, Korea

Abstract

We isolated endophytic fungi from Smilax china and Cryptomeria japonica. These fungal strains were identified based on their morphological characteristics and phylogenetic analyses of their internal transcribed spacer, large subunit rDNA, and translation elongation factor 1-α DNA. Among them, we discovered two fungi belonging to the genus Phyllosticta, which have not been previously recorded in Korea. We have described these two fungal strains, Phyllosticta ericarum 19E458 and Phyllosticta philoprina 19E012 in this study.

Figures & Tables

Fig. 1. Cultural characteristics of three endophytic fungal strains. Colonies of ericarum 19E458 grown for 7 days on potato dextrose agar (PDA) (A: Surface, E: Reverse) and malt extract agar (MEA) (B: Surface, F: Reverse), pycnidium (I) and conidia (J); Colonies of philoprina 19E012 grown for 7 days on PDA (C: Surface, G: Reverse) and MEA (D: Surface, H: Reverse), pycnidium (K) and conidia (L) (Scale bars: I, K=100 μm, J, L=10 μm).