Rhizopus soft rot caused by Rhizopus stolonifer occurred on banana (Musa sapientum) in the box at Jinju City Wholesale Market of Agricultural Products at Gyeongnam province in Korea. The infected fruits were rapidly water-soaked, softened, blackened and eventually rotted. They were infected from wounds at harvest time. The colony of the isolate from the infected fruit was white to light brown and formed many sporangiospores. The optimum growth temperature of the fungus on potato dextrose agar was 30 oC. Sporangiophores were 12~26 µm in width. Sporangia were globose or hemispheric, white at first and changed gradually to black, and 83~195 µm in size. Columella were hemispheric, and 72~125 µm in size. Sporangiospores were irregular round or oval, brownish-black and 9~20 × 7~8 µm in size. On the basis of symptom, mycological characteristics and pathogenicity to banana, the causal fungus was identified as Rhizopus stolonifer. This is the first report of Rhizopus soft rot by R. stolonifer on banana in Korea.