In some cases, the problem with the mycelium of Tricholoma giganteum is delayed mycelial growth or non-regeneration. Therefore, this study was conducted to understand the mycelial storage condition of T. giganteum and to investigate the regeneration ratio of mycelial growth. T. giganteum obtained from KACC in RDA was evaluated for its simple preservation at 10 oC and subcultured on different media. Mycelium of T. giganteum was cultured on the PBA (potato bamboo extract medium) for seven days at 30 oC. Using simple preservation method, the mycelium of T. giganteum (MKACC 50852) and Pleurotus ostreatus (Chunchu No. 2) were stored on six different media in two kinds of storage vessels (tube and vial) for a period of 1-12 months at 4 oC, 15 oC, and 25 oC storage temperatures. At 4 oC storage condition, mycelial regeneration was failed in all agar media, but was successful in the sawdust medium for 3 months. At 15 oC storage, mycelial activity was maintained up to six months. On the other hand, P. ostreatus produced well-regenerated mycelia both at 4 oC and 15 oC storage up to 12 months. In conclusion, it is estimated that the mycelia storage condition of T. giganteum must be done at 15 oC and sub-cultured within six months.