To develop artificial cultivation and improve some problems such as auto-lysis on commercialization of Coprinus comatus that has been known edible and medicinal mushroom, they were conducted for selection of superior strain, suitable culture methods for mycelial growth and fruiting, and morphological characteristics of fruit body. Strain CM 980301 of Coprinus comatus was selected as a superior strain for artificial cultivation. Wheat grain and rice straw full-grown compost media were most effective for preparation of spawn and artificial cultivation of C. comatus, respectively. Spawn running of Coprinus spp. on the rice straw full-grown compost media required to be 15 days from 24 to 28 oC. The casing layer incubation before initiation of fruit body formation, required for 13 days at same temperature for spawn running. And then require 10~11 days for initiation and 7~8 days for development of fruit body from 20 to 24 oC. The fruit body of strain CM 980301 was harvested within a week from initiation of primordium formation. The hardness of pileus and stipe that were harvested in optimal stage showed 102 to 169, and 128 to 182 g/cm ², respectively. Yields of srain CM 980301 from the rice straw full-grown compost media was 37.7 kg/3.3 m ². Weight of individual fruit body was 17.9 g in average.