Korean Journal of Mycology (Kor. J. Mycol.)
Mi-Yeong Sim 1, Jaeyoung Youm 1, Jaemin Chung 2, Byungchun Lee 2, Chang-Duck Koo 3 and Ahn-Heum Eom
1Department of Biology Education, Korea National University of Education, Chungbuk 363-791, Republic of Korea
2Department of Forest Resources Conservation, Korea National Arboretum, Gyeonggi 487-821, Republic of Korea
3Department of Forestry, Chungbuk National University, Chengju 361-763, Korea
Correspondence to eomah@knue.ac.kr
Korean Journal of Mycology (Kor J Mycol) June 30, 2010, 38(1):1-4.
https://doi.org/10.4489/kjm.2010.38.1.001
(Received June 17, 2010. Accepted June 21, 2010)
Published: June 30, 2010.
© The Korean Society of Mycology
Orchid mycorrhizal fungi(OMF) were examined in roots of the two threatened orchid species, Cypripedium japonicum and C. macranthum. The morphological characteristics of mycorrhizal colonization in the roots of two orchid species were observed. OMF colonized in the roots of two species were identified using molecular analysis. DNA from the root was extracted and amplified internal transcribed spacer(ITS) region using basidiomycete ITS primers, ITS1-OF and ITS4-OF. Four species belonging to Tulasnellaceae in roots of C. japonicum and two species of Tulasnellaceae and one basidiomycetous species was found in roots of C. macranthum.
Cypripedium japonicum, Cypripedium macranthum, OMF, Orchid mycorrhizal fungi, Peloton, Tulasnellaceae