Transplantation of matsutake-infected pine tree into a pine forest has great potential as an artificial cultivation method of Tricholoma matsutake. Matsutake-infected pine trees had previously transplanted by this research group into the experimental area from 2001 to 2004 and its survival rate determined in 2006 was 20%. For the survived samples, the characteristics of mycelial growth and the development of fairy-ring formation of T. matsutake have been evaluated until 2009. It was found that size of the indeterminate ring showed significant differences among the individual trees and varied from 4 cm x 4 cm to 52 cm x 35 cm. The variation was possibly resulted from the differences in production area of the matsutake-infected pine tree and those in site characteristics of the transplanted spots. For the characteristics of mycelial growth, it grew in the shape of a cudgel or an acute-angled ‘V’ in early stage, and then the shape became more wider and changed into an obtuse-angled ‘V’ as time passed. We expect that matsutake mushrooms may occur from the fairy-rings of some of these matsutake-infected pine trees in the autumn of 2010.