Korean Journal of Mycology (Kor. J. Mycol.)
Kyoung-In Seo 1, Kab-Yeul Jang 1, Young-Bok Yoo 1, Soon-Young Park 1, Kwang-Ho Kim 2 and Won-Sik Kong
1Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Suwon 441-707.
2Department of Crop Science, KonKuk University, Seoul 143-701, Korea
Correspondence to wskong@korea.kr
Korean Journal of Mycology (Kor J Mycol) April 1, 2011, 39(1):31-38.
https://doi.org/10.4489/KJM.2011.39.1.031
(Received 21, March 2011., Accepted 28, March 2011)
Published: April 1, 2011.
© The Korean Society of Mycology
In this study, 81 commercial strains of Pleurotus species cultivated in South Korea were analyzed with randomly amplified polymorphic DNA (RAPD) technique. Sequence characterized amplified region (SCAR) markers were developed by designing from one RAPD polymorhic band specific to Suhan strain. The SCAR primer pair ‘S-OPA13-1’ amplified a 590-bp fragment in the varieties originated from Suhan strain. The Blast search of S-OPA13-1 showed high homology to the POMFBO1 P. ostreatus cDNA clone MFB02-A05 and Laccaria bicolor S238N-H82. The results showed that this SCAR marker can clearly distinguish Suhan strains from Pleurotus spp.
Pleurotus ostreatus, RAPD, SCAR marker, Specific primers, Suhan strain