This study was carried out to investigate occurence of microbiales density and characteristics of xylanase produced by those in Janggyeong Panjeon. Cladosporium cladosporioides H1, Penicillium citreonigrum H3, Penicillilum toxicarium H4, Aspergillus versicolor H6, Acremonium alternarium H7 isolated from Janggyeong Panjeon produced xylanase, which had different production rates and specialized activities in an acidic condition. Cladosporium cladosporioides H1, Aspergillus versicolor H6, and Acremonium alternatum H7 produced xylanase at a faster rate than other fungi. A xylanase of Cladosporium cladosporioides H1 and Penicillilum toxicarium H4 showed a high thermostability in an acidic condition. As results, this study may lead to the development of a strategy for preservation of organic cultural heritages.