Jae-Young Youm 1, Han-Kyeol Han 1, Jae-Min Chung 2, Yong-Chan Cho 2, Byung-Chun Lee 2 and Ahn-Heum Eom 1*
1Department of Biology, Korea National University of Education, Chungbuk 363-791, Korea
2Department of Plant Resources Conservation, Korea National Arboretum, Gyeonggi 487-821, Korea
Correspondence to eomah@knue.ac.kr
Korean Journal of Mycology (Kor J Mycol) September 28, 2012, 40(3):132-135.
https://doi.org/10.4489/KJM.2012.40.3.132
(Received 25, July 2012., Revised 13, July 2012., Accepted 8, September 2012)
Published: September 28, 2012.
© The Korean Society of Mycology
This study was performed to isolate and identify the orchid mycorrhizal fungi (OMF) from roots of five species of terrestrial orchids in Korea; Cymbidium goeringii, Spiranthes sinensis, Calanthe discolor, Bletilla striata, Pogonia minor. DNA was extracted from isolated OMF and ITS region was amplified using primers, ITS1- OF and ITS4-OF. Four species of OMF belong to Tulasnellaceae and Sebacinaceae were identified; Tulasnella calospora, Tulasnella irregularis, Tulasnella sp., Sebacina vermifera.
Cymbidium goeringii, ITS, OMF, Orchid, Sebacinaceae, Tulasnellaceae