Han-Kyeol Han 1, Jae-Min Chung 2, Yong-Chan Cho 2, Dae-Shin Kim 3 and Ahn-Heum Eom
1Department of Biology, Korea National University of Education, Chungbuk 363-791, Korea
2Department of Plant Resources Conservation, Korea National Arboretum, Gyeonggi 487-821, Korea
3Research Institute for Hallasan, Jeju 690-816, Korea
Correspondence to eomah@knue.ac.kr
Korean Journal of Mycology (Kor J Mycol) March 31, 2013, 41(1):9-13.
https://doi.org/10.4489/KJM.2013.41.1.9
Received March 5, 2013; Revised March 19, 2013; Accepted March 20, 2013
Published: March 31, 2013.
© The Korean Society of Mycology
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
In this study, roots of Epipactis thunbergii were collected from Chujado on the north of Jeju-do. Six fungal isolates were isolated from surface-sterilized roots of the orchid and classified with groups based on morphological characteristics. Fungal DNA was extracted from each isolate and amplified ITS region using ITS1-OF/ITS4-OF primer pair. Three species of orchid mycorrhizal fungi were identified as Tulasnella calospora, Tulasnella sp. and Sebacina sp. based on molecular and morphological characteristics.
Epipactis thunbergii, Orchid mycorrhizal fungi, Sebacina, Tulasnella calospora