This study was carried out to investigated optimum mixing ratio of Korean natural Acer tegmentosum for￿ﾠ￿ﾠ production of functional oyster mushroom. Total nitrogen and carbon sources of Acer tegmentosum were 0.2% and 44.4%, respectively and C/N ratio was 234. Total nitrogen source and pH of substrate mixed with Acer tegmentosum were 2.7~2.9 and 4.8~5.0, respectively. The contents of P₂O₅, K₂O and MgO at Acer tegmentosum media were higher than that of the control. Mycerial growth was the fastest at Acer tegmentosum 10%, and slower by increase of Acer tegmentosum substrate. Yields of fruiting body was the highest to 159 g/850 mL at 10% of Acer tegmentosum and dimeter and thick of pileus were the highest, ￿ﾠ too. The L value of pileus and stipes were decreased by increase of Acer tegmentosum substrate, but there was no significant difference in the a-value and the b-value. The contents of P₂O₅ and K₂O of fruiting body were increased at Acer tegmentosum substrate, but there was no significant difference in contents of CaO, MgO and Na₂O.