Dihydroxynaphthalene (DHN) melanin is known to be present in some ascomycete fungi. To verify the type of melanin in Venturia nashicola that cause scab on pear, we investigated scytalone dehydratase (SD) gene, one of DHN melanin genes, from 11 isolates of V. nashicola from different provinces in Korea and Japan. Through PCR approach, 429 bp amplicon was produced from the 11 isolates and sequenced. All of the PCR-amplified sequences were determined as SD gene through GenBank database search. All the determined sequences were composed of an intron and two exons coding for 122 amino acids of SD. The homology of SD gene was 100% among the 11 isolates. Sequence identity of the predicted SD protein of 122 amino acids ranged 69 to 73% with other fungi. Our results proved that V. nashicola operates DHN melanin pathway.