To obtain basic data for the better use of Cordyceps pruinosa we reassessed the effect of different medium, culture method, pH, and carbon and nitrogen sources on the mycelial growth properties of four C. pruinosa isolates. The growth of mycelia differed among the four isolates depending on medium type and cultivation days. Among the tested 8 kinds of solid media, the four isolates grew well on PDA and MMMA(mushroom minimal medium agar). While, among the tested 8 kinds of liquid media, all the isolates grew well in SDYM(Sabourand’s dextrose yeast extract medium) . The isolates also grew well in the SDYM with pH from 4.0 to 9.5 without any inhibition. One isolate could best grow at pH 8 to 9.5. Regarding the ability of utilizing carbon source, the difference of mycelia growth among the isolates was the most with xylose. Regarding nitrogen source, three isolates could utilize urea which is new fact in this species. These results provide new points on the growth properties of the fungal mycelium which has not been explored before. Overall, this reassessed study concluded that it is necessary to check in advance the growth properties of mycelium when a new isolate of C. pruinosa is expected to be used for application.