Korean Journal of Mycology (Kor. J. Mycol.)
Young-Hyun You 1,3, Sang-Mo Kang 2, Yu-Mi Choi 3, Myung-Chul Lee 3* and Jong-Guk Kim 1*
1School of Life Science, Kyungpook National University, Daegu 702-701, Korea
2School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Korea
3National Agrobiodiversity Center, National Academy of Agricultural Science, RDA, Jeonju 560-500, Korea
Correspondence to mcleekor@korea.kr, kimjg@knu.ac.kr
Korean Journal of Mycology (Kor J Mycol) March 31, 2015, 43(1):71-76.
https://doi.org/10.4489/KJM.2015.43.1.71
Received: January 24, 2015; Revised: February 2, 2015; Accepted: February 3, 2015
Published: March 31, 2015.
© The Korean Society of Mycology
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Aquatic plant Hydrocharis dubia (Blume) Backer was collected from the Dalsung wetland in Daegu. Sixteen endophytic fungi with different colony morphologies were isolated from the roots of aquatic plants. Waito-c rice (WR) seedlings were treated with fungal culture filtrates (FCF) for screening plant growth-promoting activity. In the results, HD1008 strain isolated from aquatic plant showed highest plant growth-promoting activity. The FCF of HD1008 strain was analyzed using gas chromatography mass spectrometry (GC/MS) with selected ion monitoring (SIM). Analysis of the FCF of HD1008 strain found that it contained gibberellins (GA) (GA1, 1.2 ng/100 mL; GA4, 5 ng/100 mL). Phylogenetic tree of HD1008 strain was constructed by partial internal transcribed spacer (ITS) region and partial beta-tubulin gene sequences. Therefore, we describe HD1008 strain as a new gibberellin-producing Penicillium trzebinskii based on morphological and molecular characteristics.
Endophytic fungi, Fresh water, Gibberellin, Penicillium trzebinskii, Plant growth-promoting activity