Hyo-Won Choi 1*, Yong Hwan Lee 2, Sung Kee Hong 3, Young Kee Lee 1, Young Ju Nam 1, Jae Guem Lee 1 and Song Hee Han 1
1Crop Protection Division, National Academy of Agricultural Science, Wanju 565-851, Korea
2Disaster Management Division, Rural Development Administration, Jeonju 560-500, Korea
3Microbial Safety Team, National Academy of Agricultural Science, Wanju 565-851, Korea
Correspondence to hyon338@korea.kr
Korean Journal of Mycology (Kor J Mycol) June, 2015, 43(2):112-117.
https://doi.org/10.4489/KJM.2015.43.2.112
Received: May 4, 2015; Revised: June 16, 2015; Accepted: June 18, 2015
Published: June, 2015.
© The Korean Society of Mycology
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
To investigate the changes of the resistance to prochloraz of Fusarium species causing bakanae disease, Fusarium isolates were collected from various regions in Korea, and pathogenicity tests were performed using rice seeds. Minimum inhibitory concentration (MIC) and effective concentration of 50% (EC50) values of isolates were determined using the agar dilution method. High frequency distribution of MIC values of prochloraz against isolates collected in 2006~2007 and 2013~2014 years were 3.125~6.25 µg/mL and 6.25~12.5 µg/mL, respectively. The mean EC50 value of isolates increased from 0.3142 µg/mL in 2006~2007 to 0.8124 µg/mL in 2013~2014. Based on the EC50 value of isolates collected in 2006~2007, the resistant baseline of prochloraz was determined as 0.6 µg/mL. Compared with the ratio of resistant isolates in 2006~2007, the ratio of resistant isolates in 2013~2014 increased from 6.5% to 41.6%.
Bakanae disease, Fusarium fujikuroi, Prochloraz resistance