Korean Journal of Mycology (Kor. J. Mycol.)
Young-Hyun You 1*, Jong Myong Park 2, Sang-Mo Kang 3, Jong-Han Park 1, In-Jung Lee 3 and Jong-Guk Kim 4*
1Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA, Wanju 55365, Korea
2Distribution Safety Team, Safety Center, Lotte R&D Center, Seoul 07209, Korea
3School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea
4School of Life Science, Kyungpook National University, Daegu 41566, Korea
Correspondence to rocer@hanmail.net, kimjg@knu.ac.kr
Korean Journal of Mycology (Kor J Mycol) September, 2015, 43(3):200-205.
https://doi.org/10.4489/KJM.2015.43.3.200
Received: August 31, 2015; Revised: September 8, 2015; Accepted: September 16, 2015
Published: September, 2015.
© The Korean Society of Mycology
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Perilla frutescens var. japonica Hara was collected from farmland in Seongju-gun. Fifteen endophytic fungal strains with different colony morphologies were isolated from roots of P. frutescens. Waito-c rice seedlings were treated with the concentrated culture filtrates (CF) of endophytic fungi for observation of their plant growth-promoting activities. In the results, the CF of Y2H001 fungal strain promoted the growth of the waito-c rice seedlings. The phylogenetic tree of Y2H001 strain was analyzed by the combined sequences of the partial internal transcribed spacer region (ITS) and partial betatubulin gene. Molecular and morphological studies identified the Y2H001 strain as belonging to Aspergillus flavus. In gas chromatography mass spectrometry (GC/MS) analysis of the CF of Y2H001 strain, gibberellic acid (GA) was detected and quantified. Therefore, we describe Y2H001 strain as a new GA3-producing A. flavus based on morphological, molecular characteristics and analysis of secondary metabolite.
Aspergillus flavus, Endophytic fungi, Gibberellic acid, Plant growth promotion