Hyo-Ju Shin 1,3, Ock-Hee Byun 1, Yu-Jin Kim 1, Bo-Yeon Bang 1, Jung Min Park 1, Yong-Seob Jeong 3 and Dong- Hoon Bai 2*
1Korean Culture Center of Microorganisms, Seoul 03641, Korea
2Department of Food Engineering, Dankook University, Cheonan 31116, Korea
3Department of Food Science and Technology, Chonbuk National University, Jeonju 54896, Korea
Correspondence to baidh@dankook.ac.kr
Korean Journal of Mycology (Kor J Mycol) December, 2015, 43(4):247-252.
https://doi.org/10.4489/KJM.2015.43.4.247
Received: November 27, 2015; Revised: December 10, 2015; Accepted: December 14, 2015
Published: December, 2015.
© The Korean Society of Mycology
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Aronia (Black chokeberry, Aronia melanocarpa) belonging to the Rosaceae family, is native to eastern North America. Aronia contain high levels of flavonoids, mostly anthocyanins and proanthocyanidins, which are known as condensed tannins. The dominant proanthocyanidins in aronia are (-)-epicatechin and (+)-catechin. The concentration of proanthocyanidins in aronia is higher than in other berries, however due to the astringent taste it is not desirable for consumption. Therefore, the purpose of this study is to evaluate the effect of aronia on the reduction in tannins by yeast isolated from regional Jeotgal. We isolated strains of yeast with high β-glucosidase activity from Jeotgal, with the MTY2 strains exhibiting a reduction in final tannin concentration according to thin layer chromatography (TLC) analysis. MTY2 was confirmed as Kazachstania servazzii using an 18S rDNA sequence and named as K. servazzii MTY2. K. servazzii MTY2 showed most significant growth when K. servazzii MTY2 was cultured in a solution of 10% (w/v) glucose, 3% (w/v) tryptone and 0.1% (w/v) sodium chloride. According to the high performance liquid chromatography (HPLC) analysis, the (+) – catechin peak is present, but (-) – epicatechin peak was reduced at culture condition added with 10% glucose in medium.
Aronia melanocarpa, Black chokeberry, Optimal culture conditions, Proanthocyanidins, Tannins