Korean Journal of Mycology (Kor. J. Mycol.)
Hyo-Won Choi 1*, Yong Hwan Lee 2, Sung Kee Hong 3, Young Kee Lee 1, Jae Guem Lee 1 and Hyo Won Kim 1
1Crop Protection Division, National Institute of Agricultural Sciences, Wanju 55365, Korea
2Disaster Management Division, Rural Development Administration, Jeonju 54875, Korea
3Microbial Safety Team, National Institute of Agricultural Sciences, Wanju 55365, Korea
Correspondence to hyon338@korea.kr
Korean Journal of Mycology (Kor J Mycol) December, 2015, 43(4):260-266.
https://doi.org/10.4489/KJM.2015.43.4.260
Received: November 2, 2015; Revised: November 22, 2015; Accepted: November 29, 2015
Published: December, 2015.
© The Korean Society of Mycology
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
To examine the changes in resistance to benomyl of Fusarium species causing bakanae disease, Fusarium isolates were collected in Korea, and pathogenicity tests were performed using rice seeds in vitro. Minimum inhibitory concentration (MIC) and effective concentration of 50% (EC50) values of isolates were examined using the agar dilution method. High frequency distribution of MIC values to benomyl against isolates collected in 2006~2007 and 2013~2014 years were 1.5625~3.125 µg/ mL and more than 25 µg/mL, respectively. The mean EC50 value of isolates to benomyl increased from 1.6397 µg/mL in 2006~2007 to 2.4892 µg/mL in 2013~2014. Based on MIC and EC50 values of isolates, the moderate resistance of benomyl were determined as more than 25 µg/mL of MIC and less 2.4 µg/mL of EC50 value, and resistant isolates to benomyl were determined as more than 2.4 µg/mL of EC50 value. Compared with the ratio of resistant isolates in 2006~2007, the ratio of resistance isolates in 2013~2014 increased from 12.5% to 36.4%. In addition, multiple resistant isolates to prochloraz as well as benomyl increased to 20.3% in 2013~2014.
Bakanae disease, Benomyl resistance, Fusarium fujikuroi