Md. Aktaruzzaman, Joon-Young Kim, Tania Afroz, Sae-Jin Hong and Byung-Sup Kim*
Department of Plant Science, Gangneung-Wonju National University, Gangneung 25457, Korea
Correspondence to bskim@gwnu.ac.kr
Korean Journal of Mycology (Kor J Mycol) December, 2015, 43(4):277-280.
https://doi.org/10.4489/KJM.2015.43.4.277
Received: November 10, 2015; Revised: December 7, 2015; Accepted: December 12, 2015
Published: December, 2015.
© The Korean Society of Mycology
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
In August 2015, we collected samples of gray mold from sweet basil growing in Sachunmeon, Gangneung, Gangwon Province, Korea. Symptoms included extensive growth of mycelia with gray conidia on young leaves, stems, and blossoms. The pathogen was isolated from infected leaves and blossoms and the fungus was cultured on potato dextrose agar. For identification of the fungus, morphology and rDNA sequencing analysis of the fungus were performed, which confirmed its pathogenicity according to Koch’s postulates. The results of morphological examinations, pathogenicity tests, and the rDNA sequences of the internal transcribed spacer regions (ITS1 and ITS4) and the three nuclear protein-coding genes G3PDH, HSP60, and RPB2 showed that the causal agent was Botrytis cinerea. This is the first report of gray mold caused by Botrytis cinerea on sweet basil in Korea.
Botrytis cinerea, Gray mold, Pathogenicity, Sweet basil