Korean Journal of Mycology (Kor. J. Mycol.)
Indexed in SCOPUS, KCI, DOAJ
OPEN ACCESS, PEER REVIEWED
pISSN 0253-651X
eISSN 2383-5249
Research Note

Optimal Storage Condition of Clubroot Pathogen, Plasmodiophora brassicae for Artificial Inoculation

Department of Applied Biology, Chungnam National University, Daejeon 34134, Korea

Correspondence to hgkim@cnu.ac.kr

Korean Journal of Mycology (Kor J Mycol) December, 2015, 43(4):286-289.
https://doi.org/10.4489/KJM.2015.43.4.286
Received: August 4, 2015; Revised: September 10, 2015; Accepted: November 30, 2015
Published: December, 2015.
© The Korean Society of Mycology
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

ABSTRACT

Clubroot, caused by the obligate parasite Plasmodiophora brassicae, is a severe soilborne disease of Brassicaceae. Storage of clubroot gall is important for studies on pathogenicity and race identification. As the current storage method has been used for more than 100 years, a new storage method should be developed and the most efficient way maintaining pathogenicity should be determined. Effects of storage conditions with different storage periods on pathogenicity in galls of kimchi cabbage were examined in a greenhouse. The experiments were performed under six conditions and four temperatures in order to determine the most effective storage conditions for maintenance of pathogenicity. The most effective conditions for clubroot gall storage was the storage of whole gall at -70 oC or storage of filtrate at the same temperature through eight layers of gauze after homogenization of the galls.

Keywords

Clubroot gall, Optimal storage condition, Pathogenicity, Plasmodiophora brassicae, -70oC

Section