Korean Journal of Mycology (Kor. J. Mycol.)
Suyun Moon1†, Hwa-Yong Lee1,2†, Myungkil Kim3, Kang-Hyeon Ka3, Han Kyu Ko4, Jong-Wook Chung5, Chang-Duck Koo2*, Hojin Ryu1*
1Department of Biology, Chungbuk National University, Cheongju 28644, Korea
2Department of Forest Science, Chungbuk National University, Cheongju 28644, Korea
3Wood Chemistry and Microbiology Division, National Institute of Forest Science, Seoul 02455, Korea
4Forest Mushroom Research Center, National Forestry Cooperative Federation, Yeoju 12653, Korea
5Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju
28644, Korea
†Co-first author
Correspondence to koocdm@chungbuk.ac.kr, hjryu96@gmail.com
Korean Journal of Mycology (Kor J Mycol) June, 2017, 45(2):114-120.
https://doi.org/10.4489/KJM.20170014
Received: 7 March, 2017; Revised: 17 April, 2017; Accepted: 19 April, 2017
Published: June, 2017.
Ⓒ The Korean Society of Mycology
Lentinula edodes is an edible mushroom that is mainly cultivated in Asian countries. Recently, new cultivars of this mushroom have been developed in Korea; variety protection is very important, so the development of efficient molecular markers that can distinguish each variety is required. In this study, we developed cleaved amplified polymorphic sequence (CAPS) markers for the identification of L. edodes cultivars (Sanmaru 1ho and Chunjang 3ho). These markers were developed from whole genomic sequencing data from L. edodes monokaryon strain B17 and resequencing data from 10 dikaryon strains. A single nucleotide polymorphism changed in scaffold 9 POS 1630048 in Sanmaru 1ho(G→T), and in scaffold 13 POS 920681 in Chunjang 3ho (G→A). The restriction enzymes TspR I and Xho I distinguished Sanmaru 1ho and Chunjang 3ho, respectively, from other strains. Thus, we developed 2 CAPS markers for the identification of the L. edodes cultivars Sanmaru 1ho and Chunjang 3ho.
CAPS marker, Genome-wide, Lentinula edodes, Single nucleotide polymorphism