Korean Journal of Mycology (Kor. J. Mycol.)
Chang-Gi Back1†, Sun-Kyung Hwang2†, Mi jeong Park1, Young-Seok Kwon3, Hee-Young Jung4*, Jong-Han Park1
1Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal
Science, Wanju 55365, Korea
2Technology Services Division, National Institute of Horticultural and Herbal Science, Wanju 55365, Korea
3 Vegetable Research Division, National Institute of Horticultural and Herbal Science, Wanju 55365, Korea
4School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea
†These authors contributed equally to this work.
Correspondence to heeyoung@knu.ac.kr
Korean Journal of Mycology (Kor J Mycol) December, 2017, 45(4):386-393.
https://doi.org/10.4489/KJM.20170046
Received: 7 November, 2017; Revised: 25 November, 2017; Accepted: 25 November, 2017
Published: December, 2017.
Ⓒ The Korean Society of Mycology
Onion downy mildew, caused by Peronospora destructor, is a major disease in onion cultivation areas in Korea. The causal fungi were collected and analyzed based on sequence similarity and molecular phylogenetic relationships of multi-gene sequences, including the internal transcribed spacer (ITS) region. All isolates from Changnyeong-gun, Hamyang-gun, and Hapcheon-gun in Gyeongnam province, and Muan-gun, Haenam-gun, and Sinan-gun in Jeonnam province were identical in the four types of gene sequences, indicating they were genetically the same strains. In this study, a PCR method was developed based on the ITS gene sequences to amplify the specific DNA fragment for P. destructor only. The detection limit of was total genomic DNA of the P. destructor and the plant 0.7 ng/µL. Therefore, the developed PCR method could be used to detect P. destructor effectively from symptomless onion leaves.
Detection, Downy Mildew, Onion, PCR, Peronospora destructor