Korean Journal of Mycology (Kor. J. Mycol.)
Jun Young Kim1,3, Jang Si Woon1,3, Hyun Ju Kim2, Seong Hwan Kim1,3*
1Department of Microbiology, Dankook University, Cheonan 31161, Korea
2Animal and Plant Quarantine Agency, Gimcheon 39660, Korea
3Institute of Biodiversity, Dankook University, Cheonan 31161, Korea
Correspondence to piceae@dankook.ac.kr
Korean Journal of Mycology (Kor J Mycol) June, 2018, 46(2):186-192.
https://doi.org/10.4489/KJM.20180021
Received: May 16, 2018; Revised: May 25, 2018; Accepted: May 25, 2018
Published: June, 2018.
Ⓒ The Korean Society of Mycology
Rapid and accurate detection methods for Colletotrichum coccodes, an anthracnose pathogen of pepper and tomato, were developed using PCR. A specific primer set, coccoTef-F/coccoTef-R, which was constructed by analyzing tef-1α genes from 13 species and 22 strains of Colletotrichum, could specifically detect C. coccodes at a level of 10 ng by conventional PCR method and at 10 pg by real-time PCR. The PCR-based methods were also capable of detecting C. coccodes in pepper and tomato seeds artificially infected with the pathogen. The developed PCR methods can be applied for rapid and accurate inspection of C. coccodes in the seeds intended for export or import.
Anthracnose, Colletotrichum coccodes, Molecular marker