Korean Journal of Mycology (Kor. J. Mycol.)
Hye Jin Hwang 1, Hye Yeon Mun 1, Buyng Su Hwang 2, Young Ho Nam 3, and Eu Jin Chung 3,*
1Fungi Research Team, Microbial Research Department, Nakdonggang National Institute of Biological
Resources (NNIBR), Sangju 37242, Korea
2Animal&Plant Utilization Team, Animal&Plant Research Department, Nakdonggang National Institute
of Biological Resources (NNIBR), Sangju 37242, Korea
3Environmental Microbiology Research Team, Microbial Research Department, Nakdonggang National
Institute of Biological Resources (NNIBR), Sangju 37242, Korea
Correspondence to eujenee@nnibr.re.kr
Korean Journal of Mycology (Kor J Mycol) March, 2020, 48(1):15-27.
https://doi.org/10.4489/KJM.20200002
Accepted: March 26, 2020; Revised: March 25, 2020; Received: January 08, 2020
Published: March, 2020.
© 2020 THE KOREAN SOCIETY OF MYCOLOGY.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
In screening for antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) KCCM 40510 and Bacillus cereus KCTC 3624, NNIBRFG5039 was isolated from the air in Sangju-si, Gyeongsangbuk-do. Based on a high sequence similarity of the internal transcribed spacer (ITS) region, NNIBRFG5039 was determined to be closely related to Penicillium rubefaciens CBS 139145. The optimal media, initial pH, and temperature for mycelial growth and antimicrobial activity of P. rubefaciens NNIBRFG5039 were determined as follows: potato dextrose broth (PDB), pH 6.5, and 30°C, respectively. Under the optimal culture conditions, maximum mycelial growth (12.4 g L -1) and antibacterial activity (7.5 mm zone of inhibition against MRSA KCCM 40510, and 5.0 mm zone of inhibition against B. cereus KCTC 3624) were observed in a 5 L stirred-tank fermenter. We also isolated the antimicrobial compound from an ethyl acetate fraction, and its chemical structure was identified as (S)-6-hydroxymellein (1) by ESI-MS, 1H-NMR, and 13C-NMR. Consequently, the extract from P. rubefaciens NNIBRFG5039 may be used in functional materials for antimicrobial-related applications.
Antimicrobial activity, Methicillin-resistant Staphylococcus aureus, Penicillium rubefaciens, (S)-6-hydroxymellein