Benjamin Yaw Ayim 1, Kallol Das 1, Young-Je Cho 2, Seung-Yeol Lee 1,3,*, and Hee-Young Jung 1,3
1School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea
2School of Food Science & Biotechnology, Kyungpook National University, Daegu 41566, Korea
3Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Korea
Correspondence to leesy1123@knu.ac.kr
Korean Journal of Mycology (Kor J Mycol) March, 2020, 48(1):39-45.
https://doi.org/10.4489/KJM.20200004
Accepted: March 23, 2020; Revised: March 18, 2020; Received: January 15, 2020
Published: March, 2020.
© 2020 THE KOREAN SOCIETY OF MYCOLOGY.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
A fungal isolate US-18-11 was isolated from the soil in Uiseong, Korea. The mycelium growth measured after 7 days of incubation at 22℃ on malt extract agar (MEA) and oatmeal agar (OA) media was 42-43 mm and 41-44 mm in diameter, respectively. The fungal colony formed white to dull green aerial mycelia that were floccose with regular margins and olivaceous black with leaden gray patches on the reverse side. The conidia were hyaline to brown in color, ellipsoidal to ovoid, guttulate, abundant, globose, solitary, or confluent measuring 3.2- 7.2×1.1-2.3 μm. A BLAST search of the large subunit (LSU), internal transcribed spacer (ITS) region, second largest subunit of DNA-directed RNA polymerase II (RPB2) and β-tubulin (TUB2) gene sequences revealed that the isolate US-18-11 has similarities of 99, 100, 97, and 99% with those of Epicoccum draconis CBS 186.83, respectively. A neighbor-joining phylogenetic tree constructed based on the concatenated dataset of above-mentioned sequences showed that isolate US-18-11 clustered with Epicoccum draconis CBS 186.83 in the same clade. Based on the results of morphological, cultural, and phylogenetic analysis, the isolate US-18-11 was identical to the previously described E. draconis CBS 186.83. To our knowledge, this is the first report of E. draconis in Korea.
Dothideomycetes, Epicoccum draconis, Morphological characteristic, Phylogeny