Korean Journal of Mycology (Kor. J. Mycol.)
Gwang-Jae Lim 1, Kallol Das 1, Hyeong-Jin Noh 2, Seong-Keun Lim 1, Young-Je Cho 3, Seung-Yeol Lee 1,4,*, Hee-Young Jung 1,4, and Seong Hwan Kim 2
1College of Agriculture and Life Sciences, Kyungpook National University, Daegu 41566, Korea
2Department of Microbiology, Dankook University, Cheonan 31116, Korea
3School of Food Science & Biotechnology/Food & Bio-Industry Research Institute, Kyungpook National
University, Daegu 41566, Korea
4Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Korea
Correspondence to leesy1123@knu.ac.kr
Korean Journal of Mycology (Kor J Mycol) June, 2023, 51(2):111-120.
https://doi.org/10.4489/KJM.20230012
Accepted: June 28, 2023; Revised: June 26, 2023; Received: May 11, 2023
Published: June, 2023.
© 2023 THE KOREAN SOCIETY OF MYCOLOGY.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Three fungal strains designated as KNUF-23-MG32, KNUF-23-YC8, and KNUF-23-MJ82 were isolated from the abnormal symptomatic apple trees during screening fungal pathogens collected in Jeollanam-do and Gyeongsangbuk-do, Korea. These fungal strains were found to have similar cultural and morphological characteristics close to the genus Botryosphaeria. Morphological characteristics were matched with B. kuwatsukai CBS 135219 but different with B. dothidea KACC 45481 and B. sinensis HMAS 246714 T. Pathogenicity tests of strain KNUF-23-MG32 showed that this strain causes rot in Fuji apples. Molecular phylogenetic analysis based on the sequences of the internal transcribed spacer (ITS) regions, translation elongation factor 1-alpha (TEF1α), and partial β-tubulin (TUB2) showed that these strains are B. kuwatsukai. To the best of our knowledge, this is the first report of an apple ring rot fungus Botryosphaeria kuwatsukai in Korea.
Botryosphaeriaceae, Fuji apple, Malus domestica, Phylogeny